The G12 coupled thrombin receptor stimulates mitogenesis through the Shc SH2 domain
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چکیده
منابع مشابه
Phosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domain.
The SHC proteins have been implicated in insulin receptor (IR) signaling. In this study, we used the sensitive two-hybrid assay of protein-protein interaction to demonstrate that SHC interacts directly with the IR. The interaction is mediated by SHC amino acids 1 to 238 and is therefore independent of the Src homology 2 domain. The interaction is dependent upon IR autophosphorylation, since the...
متن کاملThe SH2 domain interaction landscape.
Members of the SH2 domain family modulate signal transduction by binding to short peptides containing phosphorylated tyrosines. Each domain displays a distinct preference for the sequence context of the phosphorylated residue. We have developed a high-density peptide chip technology that allows for probing of the affinity of most SH2 domains for a large fraction of the entire complement of tyro...
متن کاملSynthetic alpha-thrombin receptor peptides activate G protein-coupled signaling pathways but are unable to induce mitogenesis.
alpha-Thrombin (thrombin) stimulates phospholipase C and modulates the activity of adenylate cyclase in a number of cell types via G protein-coupled receptors. It is also a potent growth factor, notably for a line of hamster fibroblasts (CCL39 cells). Recently, predicted amino acid sequences for human and hamster thrombin receptors have been reported that display a putative thrombin cleavage si...
متن کاملStructure and specificity of the SH2 domain
In 1992 and 1993, we published two papers that described the structure of the Src homology 2 (SH2) domain of the v-Src tyrosine kinase complexed to nonspecific phosphotyrosyl peptides (Waksman et al., 1992) and to a phosphopeptide that has specificity for the Src SH2 domain (Waksman et al., 1993). These papers mark the beginnings of a research effort on the Src kinases that has resulted ten yea...
متن کاملSrc binds cortactin through an SH2 domain cystine-mediated linkage.
Tyrosine-kinase-based signal transduction mediated by modular protein domains is critical for cellular function. The Src homology (SH)2 domain is an important conductor of intracellular signaling that binds to phosphorylated tyrosines on acceptor proteins, producing molecular complexes responsible for signal relay. Cortactin is a cytoskeletal protein and tyrosine kinase substrate that regulates...
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ژورنال
عنوان ژورنال: Oncogene
سال: 1997
ISSN: 0950-9232,1476-5594
DOI: 10.1038/sj.onc.1201220